Development of Nanocomposites for Ovarian Cancer Biomarker (CA-125) Detection
Ovarian cancer (OC) presents a significant challenge due to the lack of prominent symptoms in the early stages and the limitations of existing screening procedures, resulting in high mortality rates. Hence, there is an urgent requirement to devise a highly effective label-free immunoassay for detecting cancer antigens (CA-125).
Fe-Doped, Pyrrolic-N-Modified Nanoporous Carbon Derived from ZIF-8 material.
Our study is dedicated to enhancing the sensing performance for detecting the CA-125, a goal that led us to explore the electrochemical performance and the structure of Fe-doped ZIF-8 material (Fe-ZIF-8). This unique material, when pyrolyzed, transforms into nanoporous carbon (NC) with a nitrogen-rich structure. The heteroatoms obtained by ZIF-8 carbonizing contain Fe co-doping, N, and C, which give this material higher conductivity. The nitrogen-rich structure acts as a reducing agent of metal ions to metal nanoparticles (Au@Fe-NC). A series of tests using SEM, XRD, Raman spectra, XPS, and other techniques were conducted to gain a deeper understanding of the Au@Fe-NC material. The immunosensor developed using Au@Fe-NC exhibits outstanding stability and high sensitivity to CA-125, presenting a linear dynamic range of 0.1 pg mL ⁻¹ to 1 µg mL ⁻¹ and a detection limit of 0.1 pg mL⁻¹. The proposed Au@Fe-NC-based immunosensor, with its ability to detect CA-125 in human serum samples, holds great promise for OC diagnosis.
Carboxylic carbon nanotubes or MOF (Metal-Organic Framework) materials are combined into ink and utilized to screen-print three electrodes, forming an advanced detection platform for assessing CA125 values in serum. Compared to traditional techniques, this promising approach offers a more convenient, faster, and more accurate method for monitoring the probability of cancer recurrence post-surgery.
Related publications:
- Detection of O-glycosylated CA125 by using an electrochemical immunosensor for ovarian cancer diagnosis. Link: https://doi.org/10.1007/s10800-020-01477-2
- Electrochemical detection of CA125 using thionine and gold nanoparticles supported on heteroatom-doped graphene nanocomposites. Link: https://doi.org/10.1007/s13204-021-01966-6
- rGO based immunosensor amplified using MWCNT and CNF nanocomposite as analytical tool for CA125 detection. Link: https://doi.org/10.1016/j.ab.2021.114393
- Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors. Link: https://doi.org/10.3390/s23031131
- Electrochemical immunosensing by carbon ink/carbon dot/ZnO-labeled-Ag@polypyrrole composite biomarker for CA-125 ovarian cancer detection. Link: https://doi.org/10.1016/j.bioelechem.2023.108430
- Auto-reduction of Au(III) on Fe-Doped, Pyrrolic-N-Modified nanoporous carbon derived from ZIF-8 for the electrochemical immunosensing of CA-125. Link: https://doi.org/10.1016/j.electacta.2024.144577
